English
Korean
Cell Line Development
Cell Line Development
We make it our mission to deliver stable cell clones and pools that consistently generate high yields and high- quality products of antibodies and recombinant proteins for growing biopharmaceutical industry. KCell Biosciences has extensive experiences in protein expression in mammalian cells and has established many proprietary subtypes of CHO cells to serve our mission and meet our customer’s demands.
Platform Advantages
Proven experts in Process Development
- We have been successful in delivering high quality recombinant proteins and antibodies for more than 130 contracted projects
High Efficiency and High Yield
- By integrating media formulation and process development, KCell Biosciences offers clients a more specialized approach for generating cell lines that produce the most optimal yield, quality, and stability of recombinant proteins or antibodies. Using 14-day fed-batch process, we achieve protein yields of 3 - 6 g/L, and even higher in some cases.
Documentation to Support Regulatory Filing
- KCell Biosciences provides all customers with documentation of single cell imaging, cell division, and cell growth rates during the single cell cloning process to demonstrate monoclonality for regulatory filing.
High Throughput Selection and Fast Delivery
- Integrated media formulation and process development platform enables KCell Biosciences to optimize transfection efficiency, characterize individual clones, and identify the best single cell clones for cell line development within only 3 - 5 months.
One-Stop Solution : Sequence to Commercialization
- KCell Biosciences’ stable cell line development service also offers molecular cloning of a gene sequence into an expression plasmid and media optimization services in addition to providing our customers with high-yield single cell clones. Our media optimization service enables us to be more efficient at process development and scale-up for your project needs.
Cell Line Development Procedure
<Scroll left and right to see the contents.>
| Description | Experimental content | Documentation provided |
|---|---|---|
| Plasmid Construction | Option1 : Clients provide protein sequence to KCell Biosciences for cloning into an expression plasmid Option2 : Clients provide their own plasmid |
Sequencing Report Lab Report |
| Transfection Pool | Clients receive a pool of clones that are subject to selection pressure for evaluating expression level. |
Mini pool Selection Report |
| Mini pool Selection | Clients receive 5 - 8 high yielding Mini pools that are subject to selection pressure for evaluating expression level. |
5-8 Mini pool Selection Report |
| Characterization and Identification of clones, Stability Testing, Single clone Selection |
Selection of a single cell clone that is identified based on evidence of monoclonality, product yield, growth rate, and stability. Clients can request additional single cell clones if desired. |
Single clone selection report Stability report |
| Process Scale Up (Optional) |
2 - 15 L Bioreactor Process Development, graduated scale-up, and Tech transfer |
PD Report, CMC Report Tech Transfer-related materials |
Delivery time
3 - 5 months after signing a contract
